TABLE 2.
PCR primers | |
CaMKIIαcre F | TGCCCAAGAAGAAGAGGAA |
CaMKIIαcre R | TTGCAGGTACAGGAGGTAGTC |
Tsc1loxp/loxp F | GTCACGACCGTAGGAGAAGC |
Tsc1loxp/loxp R | GAATCAACCCCACAGAGCAT |
Raptorloxp/loxp F | CTCAGTAGTGGTATGTGCTCAG |
Raptorloxp/loxp R | GGGTACGTATGTCAGCACAG |
FGF2 F | GAGATGTAGAAGATGTGACGCC |
FGF2 R | CTGTGAGGGTCGCTCTTCTC |
q-PCR primers | |
GAPDH F | TGTGTCCGTCGTGGATCTGA |
GAPDH R | TTGCTGTTGAAGTCGCAGGAG |
FGF-2 F | TCAGTAGCTGCCTGGAAACA |
FGF-2 R | AAGCCCTGCACCAAGTACAC |
FGF-1 F | ACCGAGAGGTTCAACCTGCC |
FGF-1 R | GCCATAGTGAGTCCGAGGACC |
FGF-4 F | CAAGCTCTTCGGTGTGCCTT |
FGF-4 R | GTCCGCCCGTTCTTACTGAG |
FGF-9 F | ATGCCTGTCTTCCTGTCCTG |
FGF-9 R | CGTCTGGCTCCTCTCTCTGT |
FGF-11 F | CTGTCCAAGGTGCGACTGTG |
FGF-11 R | GAACGACGCTGACGGTAGAGA |
FGF-13 F | GGCAATGAACAGCGAGGGATACTTGTACAC |
FGF-13 R | CGGATTGCTGCTGACGGTAGATCATTGATG |
FGF-14 F | GGCAACCTGGTGGATATCTT |
FGF-14 R | CCTTGCCTGCAATATAACCT |
FGF-23 F | GGTGATAACAGGAGCCATGAC |
FGF-23 R | TGCTTCTGCGACAAGTAGAC |
GFAP F | CGTTAAGCTAGCCCTGGACATC |
GFAP R | GGATCTGGAGGTTGGAGAAAGTC |
Vimentin F | TGCTTCTCTGGCACGTCTTG |
Vimentin R | GGACATGCTGTTCCTGAATCTG |