FIGURE 5.

HCD-fed Sort1 KO mice had increase bile acid synthesis and altered bile acid composition. Tissues of 1-week HCD-fed mice were used for analysis. A, liver mRNA levels. B, ileum mRNA expression. WT+C, WT plus chow diet; KO+C, knock-out plus chow diet. C and D, hepatic taurine-conjugated and free bile acids (BA) determined by metabolomics analysis. Values in WT mice were set as 1. AUC, area under the curve; T, total. E, bile acid composition in gallbladder bile was analyzed by LC-MS/MS. F, liver 7α-HO-3-oxo-4-cholestenoate detected by metabolomics analysis. The value in WT was set as 1. All results are expressed as mean ± S.E. n = 5–6. * indicates statistical significance, versus WT mice on same diet. # indicates statistical significance, versus chow-fed mice of the same genotype.