FIGURE 6.

Exogenous p150 expression suppresses cell invasiveness in transformed MCF10A Src-ER cells. A, MCF10A Src-ER cells transduced with either GFP or GFP-p150 were fixed and stained with DAPI. Scale bar, 10 μm. B, total cell lysates from MCF10A Src-ER cells transduced with either GFP or GFP-p150 were immunoblotted with antibodies against GFP, p150, and α-tubulin. C, total cell lysates from MCF10A Src-ER cells transduced with either GFP or GFP-p150 and treated for 48 h with either EtOH or 1 μm 4-OHT were immunoblotted with antibodies against GFP, p150, pSrc (Tyr⁴¹⁶), and α-tubulin. D, MCF10A Src-ER cells transduced with either GFP or GFP-p150 were treated for 48 h with either EtOH or 1 μm 4-OHT. Equal numbers of cells were spread onto Matrigel-coated membrane filters. Invading cells were counted. The individual values (gray circle), means (black box), and S.D. of means of invading cells from a field are shown. The means ± S.D. were derived from three biological replicates. E, equal numbers of U2OS cells transduced with either GFP or GFP-p150 were spread onto membrane filters. Migrating cells were counted. The individual values (gray circle), means (black box), and S.D. of means of migrating cells from a field are shown. The means ± S.D. were derived from two biological replicates.