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. 2016 Sep 22;292(1):185–195. doi: 10.1074/jbc.M116.743724

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FIGURE 8. — Water permeability of the R153A/S229D double mutant in 2 mm Ca²⁺ did not depend on amount of cRNA injected. AQP0 P_f (minus uninjected oocyte P_f) doubled with a doubling of the amount of cRNA in both 2 mm Ca²⁺ and 1 mm EGTA. R153A/S229D double mutant P_f (minus uninjected oocyte P_f) in 1 mm EGTA doubled, but the P_f in 2 mm Ca²⁺ did not change. *, identical to 2 times 10 ng of cRNA P_f (minus uninjected oocyte P_f) (p = 1.00). Δ, identical to uninjected (p = 1.00). Uninjected oocyte P_f = 7.4 μm/s.