FIGURE 6.

NRF2 is neither recruited to the IL11 promoter nor interacts with Fra-1. A, NRF2 is not recruited to the IL11 promoter after 1,2-NQ stimulation. HepG2 cells were stimulated with the indicated concentrations of 1,2-NQ (50 μm) for 2 h, and then cells were subjected to ChIP assays using control Ig or anti-NRF2 antibody. Immunoprecipitated fragments of the NQO1 and IL11 promoters or an unrelated region of NQO1 were analyzed by qPCR. Enrichment of the promoter fragments was expressed as a percentage of input. Results are mean ± S.D. (error bars) of triplicate samples. *, p < 0.05; ***, p < 0.001; ns, not significant versus untreated cells. Results are representative of four independent experiments. B, Fra-1 interacts with ERK2, but not NRF2. HEK293T cells were transiently transfected with the indicated expression vectors, and lysates were immunoprecipitated (IP) with the indicated antibodies. Co-immunoprecipitated proteins were analyzed by immunoblotting with the indicated antibodies. Expression of transfected proteins was verified by immunoblotting with the indicated antibodies using total cell lysates. Arrowheads and asterisks, phosphorylated and unphosphorylated Fra-1, respectively. C and F, control Ig and anti-FLAG antibody, respectively. Results are representative of three independent experiments.