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. 2016 Nov 28;292(1):217–228. doi: 10.1074/jbc.M116.763664

FIGURE 3.

FIGURE 3.

Comparison of DNA binding by Teb1, TEB, Rpa1, RPA, Rlp1, and RTT. A, domain structures for Teb1, Rpa1, Rlp1, Teb2, and Teb3 and complexes formed by co-assembly that were assayed for DNA binding affinity. B, colloidal Coomassie staining after SDS-PAGE of proteins and complexes used for DNA binding assays. *, a background protein in the Teb2-Teb3 purification. Enhanced contrast for the bottom of the gel is shown below the main gel panel. C, DNA binding by Teb2-Teb3, Teb1, TEB, Rpa1, RPA, Rlp1, and RTT. A fixed concentration of the indicated end-labeled ssDNA oligonucleotide (∼10 pm) was incubated with protein or protein complex added at steps of final concentration over the indicated range of 0.1–62.5 or 1–625 nm.