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. 2016 Nov 21;292(1):251–263. doi: 10.1074/jbc.M116.752816

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FIGURE 6. — Two-dimensional ¹H-¹⁵N HSQC spectra obtained on a ¹⁵N-labeled CaM-N at 60 μm as titrated with Akt(PHD). Spectra in black and red were obtained for CaM-N in the free state and in complex with excess Akt(PHD) to ensure saturation (1:6 ratio). The spectrum in green was obtained for the CaM-N·Akt(PHD) complex after diC₄-PI(3,4,5)P₃ was added at 2:1. The ¹H-¹⁵N signals of CaM-N do not revert back to the positions observed for free CaM-N, indicating that Akt(PHD) is still bound to CaM-N upon titration of diC₄-PI(3,4,5)P₃. Signals denoted by asterisks are folded in the spectrum by 20 ppm.