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A, elution profiles of MSP1D1/POPC/POPS/DPPI(3,4,5)P3 nanodisc, Akt(PHD), and their mixture at 1:1 on a HiLoad Superdex 200 column. The elution volume of nanodisc is consistent with formation of a homogeneous nanodisc. B, fractions were analyzed by SDS-PAGE. Notice that Akt(PHD) elutes with nanodisc. Unbound Akt(PHD) is also detected. M, marker; L, load.
