TABLE 1.

Thermodynamic parameters for Akt(PHD) and Akt(PHD)-CaM binding to diC₄-PI(3,4,5)P₃ and nanodisc

Lipid/membrane	Kd	ΔH	−TΔSa	ΔG	n
	nm	kcal/mol	kcal/mol	kcal/mol
Akt(PHD)
diC4-PI(3,4,5)P3	13,000	−2.7 ± 0.3	−3.8 ± 0.2	−5.83	0.92 ± 0.06
Nanodisc	19	−5.5 ± 0.2	−5.0 ± 0.5	−10.5	0.57 ± 0.02
Akt(PHD)-CaM
diC4-PI(3,4,5)P3	8000	−3.1 ± 0.2	−3.8 ± 0.3	−6.90	1.0 ± 0.08
Nanodisc	200	−4.9 ± 0.1	−4.1 ± 0.2	−9.01	0.63 ± 0.01

^a Data were collected at 20 or 30 °C. Experiments were collected in triplicates.