FIGURE 4.

Antisera from cyclically permuted gp120 trimers elicit broad cross-reactive neutralizing responses. TZM-bl ID₅₀ values (neutralizing titers) for week 20 antisera from guinea pigs in TZM-bl cell-based neutralization assays. The neutralizing activity of antisera from each group of guinea pigs was tested against Tier 1 and Tier 2 HIV-1 isolates from a global panel and additional Tier-2 isolates, which included viruses from clades A, B, C, and CRFs. The neutralization titers are corrected for their respective preimmune backgrounds. The color code indicates the following: ID₅₀ ≤ 60 or ≤ 2-fold of MuLV neutralizing titers are in white, ID₅₀ from 60 to 90 are in yellow, ID₅₀ from 90 to 500 are in orange, and ID₅₀ >500 are highlighted in red. Group number and corresponding immunogen used for vaccination are indicated in the first column.