FIGURE 4.

¹³C labeling from ¹³CO₂ of TG molecular species during a time course of nitrogen starvation. Labeled TG molecular species were separated chromatographically and isolated, and the ¹³C content was measured by LC-MS. Data are shown as ¹³C labeling in TG molecular species (A) and the relative amount of ¹³C and unlabeled ¹²C in the total TG pool (B). The numbers indicate the quantity of ¹³C (dark gray) and ¹²C (light gray) in the TG pool as a percentage of the total dry weight of algae. Error bars represent S.D.; n = 3.