FIGURE 7.
Incorporation rates of de novo synthesized FA in nine lipid classes after 2 days of nitrogen starvation. Cultures were labeled with D2O after 2 days of nitrogen stress, and acyl chain labeling was analyzed in the lipidome at 3, 6, 9, 12, and 24 h to determine incorporation rates. Lipid extracts were separated by TLC before synthesis of methyl esters and GC-MS analysis of TAG, sterol esters (St-E), MGDG, DGDG, TGDG, PG, PS, PC, and PE. Shown are the rates of incorporation of de novo synthesized oleoyl (18:1), palmitoyl (16:0), and palmitoleoyl (16:1) acyl chains into specified lipid classes. Error bars represent S.D.; n = 4 independent cultures.