FIGURE 11.

MEG3 could associate with the specific regulatory regions of CDH1, miR-200a, and miR-200c genes in A549 cells. ChIRP analyses of MEG3 on the regulatory regions of CDH1 (A), miR-200b/200a/429 (B), miR-200c/141 (C), and GAPDH genes (D) in A549 cells are shown. The cells were infected with the control retrovirus or the retrovirus expressing MEG3 with or without treatment of TGF-β. The cross-linked cell lysates were incubated with biotinylated DNA probes against MEG3 lncRNA, and the binding complexes were recovered by streptavidin-conjugated magnet beads. QPCR was performed to detect the enrichment of the specific regulatory regions that associated with MEG3 lncRNA. (*, p < 0.01 compared with control; **, p < 0.05 compared with control; ***, p < 0.01 comparing to the sample without TGF-β treatment).