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. Author manuscript; available in PMC: 2017 Mar 28.
Published in final edited form as: J Am Chem Soc. 2016 Sep 20;138(38):12629–12635. doi: 10.1021/jacs.6b07680

Figure 1.

Figure 1

An in vivo covalent chemical capture and mass spectrometric-based approach for the identification of the cellular targets of transcriptional activators. Live yeast expressing Bpa-containing activators (blue and red) are irradiated with UV light to covalently capture the spectrum of protein binding partners that activators directly contact in cells. Following cell lysis, affinity chromatography and immunoprecipitation are used to enrich for cross-linked complexes. The purified products are then subjected to mass spectrometric-based analysis such as Mud-PIT, thus allowing for the concurrent verification of previously identified activator targets (green) and the discovery of novel binding partners (orange).