Fig. 2.

A: Agnoprotein from JCV, BKV, and SV40 forms highly stable dimers and oligomers in vitro. Agnoprotein was fused to MBP, expressed in E. coli, and purified as described previously (Saribas et al., 2011). MBP and MBP-Agnoprotein were analyzed by SDS-10% polyacrylamide gel electrophoresis followed by Coomassie blue staining. The positions of monomers, dimers, and oligomers are indicated by arrows. B: Agnoprotein forms stable dimers and oligomers in vivo. Whole cell extracts were prepared from SVG-A cells infected with JCV Mad-1 strain at the 21st day post-infection and analyzed by Western blotting using primary anti-agnoprotein polyclonal (Del Valle et al., 2002) antibody. The positions of monomer, dimer, and oligomers are indicated by arrows. Inf., infection.