Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Calculated KIE for SLO double mutant L546A/L754A as a function of the proton donor-acceptor equilibrium distance and associated frequency Ω. The curves are labeled according to R0 given in Å. In this model, the effective mass of the proton donor-acceptor mode is M = 100 amu, the reorganization energy is λ = 45.6 kcal/mol, and the reaction free energy is ΔG° = −5.4 kcal/mol. The shaded area of the plot corresponds to the experimental range of the observed KIE between 30 and 35°C
