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In vitro degradation assay of MBP-YbaB. Degradations of MBP-YbaB by ClpYQ at 37 °C were determined by anti-MBP antibody and analyzed by Image J. A, Coomassie blue staining of purified MBP-YbaB, ClpY and ClpQ. B, SDS-PAGE of YbaB degradation by ClpYQ. (C) YbaB degradation by ClpYQ determined by Anti-MBP Western blotting. Means and standard deviations (error bars) were taken from three independent analyses. The asterisk indicates the significant difference by Student's t test (p < 0.05).
