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. 2016 Nov 16;16(1):23–38. doi: 10.1074/mcp.M116.061200

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Fig. 5. — A, Western blot of whole cell extracts (WCE) and chromatin extracts (EC or TC) of epimastigotes (Epi) and trypomastigotes (Trypo) forms using antibodies against the indicated proteins. Proteins expressed preferably in trypomastigotes, epimastigotes and expressed in both forms (common) are indicated. Antibodies against the Orc1/Cdc6 present mainly in the chromatin of epimastigotes were used as a control. B, T. cruzi epimastigote or trypomastigote cells were submitted to a chromatin immunoprecipitation assay using antibodies or pre-immune serum as indicated. After immunoprecipitation, cross-links were reversed, and DNA was extracted and quantified. Percentage of the immunoprecipitated DNA related to input after subtraction of percentage of DNA immunoprecipitated by preimmune serum was calculated. Graph indicates the ratio of these values between epimastigote and trypomastigote cells.