Table I. Top 10 up- and downregulated proteins in asthenozoospermia samples compared to NZS in human sperm cell samples. Peptide count is the total peptides found for corresponding proteins and unique peptides is the number of unique peptides out of the total peptides found. Confidence score, ANOVA p value, maximum fold change, disease class of the highest and lowest mean condition and full name of the protein are also reported in separate columns.

Accession	Peptide count	Unique peptides	Confidence score	ANOVA (p)	Max fold change	Highest mean condition	Lowest mean condition	Protein Name
Top 10 Downregulated proteins in Asthenozoospermia in human sperm cell samples
P13727	5	4	29.3207	5.89E-06	79.40088	Normal	Astheno	Bone marrow proteoglycan
P59665;P59666	7	6	51.7033	0.125966	21.76551	Normal	Astheno	Neutrophil defensin 1
Q9BYX7	10	2	62.5462	6.93E-13	17.17476	Normal	Astheno	Putative beta-actin-like protein 3
P62736;C9JFL5;F6QUT6;F6UVQ4;P63267;P68032	29	3	207.7618	5.79E-07	15.40699	Normal	Astheno	Actin, aortic smooth muscle
Q92766	7	3	39.7539	1.78E-06	8.022462	Normal	Astheno	Ras-responsive element-binding protein 1
E9PN67;C9J066;Q8N4C6	5	4	28.2629	7.18E-11	6.170855	Normal	Astheno	Ninein
H0YCJ7;E9PJH4;E9PK82;E9PL09;E9PPU1;E9PQ96;F2Z2S8;H0YEU2;H0YF32;P23396	3	2	15.195	0.025676	5.929772	Normal	Astheno	40S ribosomal protein S3 (Fragment)
Q12906;K7ELV3	7	2	42.8884	7.07E-07	5.785136	Normal	Astheno	Interleukin enhancer-binding factor 3
Q8NCQ7;A0A0A0MT24	3	2	16.7889	1.45E-06	5.648057	Normal	Astheno	Protein PROCA1
A6NNN6;E7ETA6;Q15154	7	2	35.0439	0.006358	5.557926	Normal	Astheno	Pericentriolar material 1 protein
Top 10 Upregulated proteins in Asthenozoospermia in Human sperm cell samples
Q9NY65;C9J2C0;V9GZ17	37	3	181.908	6.35E-06	19.69428	Astheno	Normal	Tubulin alpha-8 chain
A7MCY6	3	2	14.8265	8.59E-05	17.9325	Astheno	Normal	TANK-binding kinase 1-binding protein 1
P20151	11	5	67.5844	1.46E-07	10.12548	Astheno	Normal	Kallikrein-2
Q9H0I9	4	3	30.5099	0.001624	9.963466	Astheno	Normal	Transketolase-like protein 2
Q05639	10	2	63.3446	8.28E-05	8.014055	Astheno	Normal	Elongation factor 1-alpha 2
G3V1V0;B7Z6Z4;F8VPF3;F8VZU9;F8W180;F8W1R7; G3V1Y7;G8JLA2;J3KND3;P60660	4	2	32.5303	0.011136	7.863896	Astheno	Normal	Myosin light polypeptide 6
Q15722	2	2	9.3132	0.935433	7.747275	Astheno	Normal	Leukotriene B4 receptor 1
P28340;M0R2B7	4	3	21.6549	2.12E-07	7.521456	Astheno	Normal	DNA polymerase delta catalytic subunit
P04792;C9J3N8;F8WE04	8	3	70.4424	5.95E-08	7.519383	Astheno	Normal	Heat shock protein beta-1
P32119;A6NIW5	13	4	83.2372	0.000142	6.928101	Astheno	Normal	Peroxiredoxin-2