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. 2016 Nov 16;16(1):73–85. doi: 10.1074/mcp.M116.061531

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Fig. 2. — Identification of Cdc42 interaction partners with LF-qGAP from mouse cerebellum. (A) Protein lysates from mouse cerebellum (n = 3 independent samples) were incubated with Cdc42GTPγS or Cdc42GDP coupled to beads. Bound proteins were eluted and analyzed via mass spectrometry in triplicates. Differences in intensities between runs (Log2 Cdc42GTPγS/Cdc42GDP) and statistical significance (-Log10(p value t test)) were calculated. (B) Specific interactors are distinguished from background proteins based on a combination of the log2 fold change and the t test p value (significantly different proteins in orange), Cdc42GTPγS (right) or Cdc42GDP (left) (colored in red). (C) We identified more interaction partners for LF-qGAP (44 proteins) than for SILAC-qGAP (25) with a significant overlap (15).