Scheme of the DML method and nano-LC-MS/MS analysis
followed for the proteomics study. HT-29 cells were
incubated with different concentrations (GI50, TGI, LC50) of two
polyphenols (CA, CS) or vehicle for 2, 6. or 24 h. After protein
extraction and enzymatic digestion, peptides from control and
treated samples were labeled as “light” and
“medium” respectively, and mixed 1:1 prior to
nano-LC-MS/MS analysis.