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. 2016 Nov 10;16(1):8–22. doi: 10.1074/mcp.M116.061481

Fig. 2.

Fig. 2.

Scheme of the DML method and nano-LC-MS/MS analysis followed for the proteomics study. HT-29 cells were incubated with different concentrations (GI50, TGI, LC50) of two polyphenols (CA, CS) or vehicle for 2, 6. or 24 h. After protein extraction and enzymatic digestion, peptides from control and treated samples were labeled as “light” and “medium” respectively, and mixed 1:1 prior to nano-LC-MS/MS analysis.