Skip to main content
. 2016 Oct 11;187(2):213–224. doi: 10.1111/cei.12868

Figure 3.

Figure 3

Pretransfer treatment with A213 suppresses production of various cytokines by muscarinic acetylcholine receptor (M3R)‐reactive CD4+ T cells. (a) Comparison of interferon (IFN)‐γ and interleukin (IL)‐17 production from splenocytes treated with M3R peptides obtained from immunized M3R–/– mice treated with A213 and phosphate‐buffered saline (PBS). Data are mean ± standard error of the mean (s.e.m.) of three mice per group (representative data of two independent experiments). *P < 0·05 (Student's t‐test) not detected (n.d.). (b,c) Criss‐cross co‐culture assay using CD4+ T cells and CD11c+ cells (at a ratio of 5 : 1) obtained from immunized M3R–/– mice treated with A213 or PBS under stimulation with M3R peptides. After 72 h of co‐culture, IFN‐γ (b) and IL‐17 (c) levels in the culture supernatants were analysed by enzyme‐linked immunosorbent assay (ELISA). Data are mean ± s.e.m. of three mice per group (representative data of two independent experiments). *P < 0·05; **P < 0·005 (Student's t‐test) not detected (n.d.).