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. 2016 Oct 11;187(2):213–224. doi: 10.1111/cei.12868

Figure 6.

Figure 6

Effects of A213 on the induction of T helper type 1 (Th1), Th17 and Th2 cell differentiation in vitro. (a) CD4+ T cells from naive wild‐type mice were cultured with interleukin (IL)‐12 and anti‐IL‐4 [T helper type 1 (Th1)‐promoting condition]. Four days later, live T cells, expression levels of T‐bet and interferon (IFN)‐γ were analysed by flow cytometry. Data are mean ± standard error of the mean (s.e.m.) of three mice per group (representative data of two independent experiments). *P < 0·05 (Kruskal–Wallis test). (b) CD4+ T cells were cultured with IL‐6, transforming growth factor (TGF)‐β, anti‐IL‐4 and anti‐IFN‐γ (Th17‐promoting condition). Four days later, live T cells, expression levels of retinoic acid‐related orphan receptor‐gamma t (RORγt) and IL‐17 were analysed by flow cytometry. Data are mean ± s.e.m. of three mice per group (representative data of two independent experiments). *P < 0·05 (Kruskal–Wallis test). (c) CD4+ T cells were cultured with IL‐4, IL‐2 and anti‐IFN‐γ (Th2 promoting condition). Six days later, live T cells, expression levels of GATA binding protein 3 (GATA3) and IL‐4 were analysed by flow cytometry. Data are mean ± s.e.m. of three mice per group (representative data of two independent experiments). *P < 0·05 (Kruskal–Wallis test).