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. 2017 Jan 5;3(1):vew035. doi: 10.1093/ve/vew035

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© The Author 2017. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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In vivo detection of the An. sinensis flavivirus-derived EVE. (A) EVE detection in genomic DNA from the Korean strain of An. sinensis. Lane 1, size marker; lane 2, amplified genomic DNA from a pool of 10 An. minimus adult females; lane 3, amplified genomic DNA from a pool of 10 An. minimus adult males; lane 4, amplified genomic DNA from a pool of 10 An. sinensis adult females; lane 5, amplified genomic DNA from a pool of 10 An. sinensis adult males; lane 6, amplified DNA from a pool of 10 An. stephensi females; lane 7, NTC. (B) EVE detection in total RNA from the Korean strain of An. sinensis. Lane 1: size marker; lanes 2 and 3, amplified cDNA from pools of five adult females; lanes 4 and 5, amplified cDNA from pools of five adult males; lanes 6 and 7, amplified cDNA from pools of five L₄ larvae; lane 8, amplified DNA from a pool of ten females; lane 9, amplified cDNA from a pool of five An. stephensi females; lane 10: DNA contamination control (no reverse transcription) using the same pool of five adult females as lane 2; lane 11, NTC. First row, EVE; second row, RPS7 (control gene). The RPS7 target DNA sequence includes an intron, so that DNA contamination is expected to result in a larger PCR product.