Table 2.
Carotenoids separated on a reverse‐phase C30 HPLC system and spectral characteristics used in identification from photodiode array detection
| Carotenoid | Spectral characteristics(nm λ max) | Retention time(min) |
|---|---|---|
| Neoxanthin | 466 | 6.99 |
| Violaxanthin | 433 | 7.68 |
| Chlorophyll b | 465, 471 | 10.6 |
| Phytoene 1 | 284–286 | ~10.8 |
| Lutein | 442, 450 | ~11 |
| Chlorophyll a | 430 | 12.73 |
| Zeaxanthin | 430 | 13.33 |
| Phytoene 2 | 284–286 | ~13.5 |
| Phytofluene 1 | 343/348 | ~14.3 |
| Phytofluene 2 | 343 | ~15.1 |
| Others (unknown) | 441, 443 | 15.96, 16.44 |
| α‐Carotene | 407 | 17.03 |
| β‐carotene | 450 | ~17.1, 17.6 |
| Cis‐Lycopene | 471 | ~22.2 |
| Trans‐Lycopene | 471 | ~24 |