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. 2004 Oct;78(20):11016–11029. doi: 10.1128/JVI.78.20.11016-11029.2004

FIG.5.

FIG.5.

Stable expression of Hsp16 in H9 and CEM-SS cells prior to HIV infection reduces viral replication and HIV-induced cytopathicity in a Vpr-dependent manner. (A) Western blots show high levels of Hsp16 in CD4+ H9 and CEM-SS cells. Lane 1, mock-infected cells; lane 2, HIV-infected cells carrying plasmid pcDNA3.1; lane 3, HIV-infected cells expressing hsp16. Blots show 16-kDa bands reacted to anti-Hsp16 antibody. LC, protein loading control. (B) Stable expression of Hsp16 in H9 and CEM-SS cells blocks the gross enlargement of cells typically induced by Vpr-positive HIV infection (29). Images were taken with equal amount of cells. Gross enlarged cells are of typical effect caused by Vpr (29). (C) Stable expression of Hsp16 in CD4+ H9 and CEM-SS cells inhibits viral replication in Vpr-positive HIV infection; Hsp16 has no effect on viral replication in Vpr-negative HIV infection.