TABLE 1.

Fission yeast strains, human cell lines, plasmids, and HIV-1 viral stocks

Strains, cell lines, plasmids, and viral stocks	Genotype and characteristics	Source or reference
S. pombe strains
SP223	Wild type, h−, ade6-216, leu1-32, ura4-294	ATCC
Q812	Wild type, h−, leu1-32, ura4-294	49
Q1649	Wild type, h−, leu1-32, ura4-294, gfp-hsp16	49
RE076	SP223 with single copy of F34Ivpr integrated at ura4 locus; used for measuring Vpr-induced cell cycle G2 delay	19
RE007	SP223 with single copy of vpr integrated at ura4 locus; used for determining Vpr-induced cell killing	This study
RE078	SP223 with single copy of gfp-vpr integrated at ura4 locus; used for determining nuclear localization of Vpr	This study
Mammalian cell lines
HeLa	A cervical epithelial cell line	ATCC
H9	CD4+ cells derived from PBMC for high-yield permissive growth with HIV-1	44
CEM-SS	CD4+ cells derived from human T4-lymphoblastoid cells	37
293T	An embryonic kidney fibroblast cell line	ATCC
293T-632	A 293T cell line expressing pVgRxR and pCMV-EBNA1	66
Plasmids
pYZIN	Fission yeast expression vector with an inducible nmt1 promoter and a leu1 selectable marker	63
pYZ3N	Same as pYZ1N but with a 5′ GFP-tag	63
pSF173	Fission yeast expression vector with an inducible nmt1 promoter and a 5′ HA-tag, ura4 selectable	ATCC
pZY1	Mammalian expression vector with a muristerone A-inducible promoter; geneticin-resistant	66
pZH1	Mammalian expression vector with a muristerone A-inducible promoter; hygromycin-resistant	66
pCDNA3.1	Mammalian expression vector with a CMV promoter; hygromycin-resistant	Invitrogen
HIV-1 viral stocks
HIV-1LAI Vpr (+)	HIV-1 viral laboratory (subtype B) strain LAI	4
HIV-1NL4-3 Vpr (+)	Vpr-positive NL4-3-based HIV-1 vector pNLHX	8
HIV-1NL4-3 Vpr (−)	Same as above; vpr gene is mutated at the start codon from ATG to GTG	43
HIV-1 (VSV-G) Vpr (+)	Vpr-positive NL4-3-based HIV-1 vector. HIV-1 env gene is replaced by VSV-G gene	8
HIV-1 (VSV-G) Vpr (−)	Same as above; vpr gene is mutated at the start codon from ATG to GTG	43