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. 2016 Jul 8;8(1):44–55. doi: 10.1111/jdi.12540

Figure 5.

Figure 5

Chronic mild hyperglycemia slightly enhanced α‐cell replication with no excess glucagon secretion. (a) Representative islets stained with antibodies against glucagon (green) and Ki67 (red) of normal rats (Control; n = 5), streptozotocin (STZ)‐treated rats at day 2 (STZ‐2d; n = 5), and rats at day 5, day 15 and day 60 with no treatment (HG‐5d, HG‐15d, HG‐60d; n = 4), mild hyperglycemia incubation (mHG‐5d, mHG‐15d, mHG‐60d; n = 4) and euglycemia incubation (NG‐5d, NG‐15d, NG‐60d; n = 4). The arrows: glucagon+ cells showing Ki67 expression. Nuclei were labeled with 40′,6‐diamidino‐2‐phenylindole. Scale bars, 50 μm. (b) Quantification of glucagon+ cells co‐expressing Ki67. The results are presented as the mean ± standard deviation. *P < 0.05, **P < 0.01. Quantification of (c) α‐cell number was shown in and (d) serum glucagon level. The results are presented as the mean ± standard deviation. *P < 0.05, **P < 0.01.