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. 2004 Oct;78(20):11303–11312. doi: 10.1128/JVI.78.20.11303-11312.2004

FIG. 1.

FIG. 1.

PRP4 binds specifically with HIV-2 Gag. (A) In vitro binding of PRP4 to HIV-1 and HIV-2 Gag proteins. In vitro-transcribed and -translated HIV-1 Gag (lanes 2 to 4), HIV-2 Gag (lanes 6 to 8), or gelsolin (lanes 10 to 13) was analyzed by GST fusion pull-down assays for the ability to bind GST, GST-PRP4, GST-Gag1, or GST-Gag2 immobilized on glutathione-Sepharose beads. Inputs were 10% of the amount of HIV-1 Gag (lane 1), HIV-2 Gag (lane 5), or gelsolin (lane 9) used in each assay. (B) PRP4 cellular lysate binding to HIV-2 Gag protein. In vitro-transcribed and -translated HIV-2 Gag (lane 2) or gelsolin (lane 4) was analyzed by pull-down assay for the ability to bind PRP4 protein extract from transfected 293T cells immobilized on protein A-protein G beads. Inputs were 10% of the amount of HIV-2 Gag (lane 1) or gelsolin (lane 3) used in each assay. The positions of marker proteins are indicated in kilodaltons.