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. 2004 Oct;78(20):11303–11312. doi: 10.1128/JVI.78.20.11303-11312.2004

FIG. 7.

FIG. 7.

PRP4 and HIV-2 Gag protein interact in vivo. Cos-1 cells were transfected with an HIV-2 proviral construct (lane 1), a full-length PRP4 expression vector incorporating an HA tag (lane 2), or both (lane 3). Cell pellet extracts were blotted either with anti-p26 (lane 1) or anti-HA (lane 2) antibody to determine the amount of protein present. Cells were cotransfected with these plasmids, and HIV-2 Gag protein was immunoprecipitated with anti-p26 and then blotted with anti-HA monoclonal antibody to detect PRP4 (lane 3). Marker lane proteins are indicated in kilodaltons.