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. 2017 Jan 6;12(1):e0169717. doi: 10.1371/journal.pone.0169717

Fig 1. Histological analyses of the mouse lumbar spine and a mechanical stress loading device.

Fig 1

The application of mechanical stress to the mouse LF using a novel device. (A) HE and (B) EVG staining of the spine sections. (C) Photographs and (D) schematic illustrations of the device. (E) A coronal CT image showing that the iliac crest line corresponds to the L5-L6 disc level. (F) Sagittal CT images showing that mechanical stress was consistently applied at the L5-L6 level. Scale bars (A): 300 μm; insets: 50 μm; (B): 300 μm; insets: 100 μm. LF, ligamentum flavum; Ver, vertebral body; Du, dural tube; SAP, superior articular process; IAP, inferior articular process; La, lamina.