Table 5. Steroid ratios for detection and identification of a faecal input applied on archaeological soil samples.
No. | Ratio | Dormagen | Inden | Düren-Arnoldsweiler | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Cess-pit | Stable | Control | Sewer | Control | Well | |||||||
Drain (brown filling) | Drain (green filling) | Stable area | 70 cm depth | 80 cm depth | LBK | Bronze Age | Iron Age | |||||
Ratios for mere detection of faecal matter | ||||||||||||
I | (coprostanol + epicoprostanol) / (5α-cholestanol + coprostanol + epicoprostanol) † | 0.57 | 0.32 | 0.26 | 0 | 0 | 0.43 | 0.30 | 0 | 0.89 | 0.70 | 0.37 |
II | (5β-stigmastanol + epi-5β-stigmastanol) / (5α-stigmastanol + 5β-stigmastanol + epi-5β-stigmastanol) ‡ | 0.37 | 0.35 | 0.15 | 0.11 | 0.14 | 0.37 | 0.55 | 0.15 | 0.31 | 0.58 | 0.18 |
Ratios for identification of faecal matter | ||||||||||||
V | coprostanol / (coprostanol + 5β-stigmastanol) x 100% § | 46% | 29% | 39% | 0% | 0% | 28% | 18% | 0% | 40% | 12% | 42% |
VI | epi-5β-stigmastanol / 5β-stigmastanol + epicoprostanol / coprostanol | 0.58 | 1.74 | 1.50 | - | - | 0.50 | 1.42 | - | 0.41 | 2.90 | 1.04 |
Bile acid ratios | ||||||||||||
DCA / LCA | 11 | 5.6 | 9.3 | 6.7 | 8.9 | 10 | 1.7 | 13 | 7.4 | 14.1 | 6.3 | |
DCA / CDCA | 8.2 | 6.1 | 9.2 | 9.1 | 9.4 | 11 | 4.5 | 3.2 | 37 | 24 | 204 | |
CDCA / LCA | 1.3 | 0.9 | 1.0 | 0.7 | 1.0 | 0.9 | 0.4 | 4.1 | 0.2 | 0.6 | 0.03 | |
HDCA / LCA | 4.6 | 1.2 | 4.3 | 1.3 | 3.5 | 14 | 0.8 | 11 | 9.5 | 11 | 1.1 |
All ratios calculated from the means of n = 3 laboratory replicates; when steroid contents were below the routine quantification limit, they were treated as zero for the calculation of the ratios.
References for the used ratios:
† Bull et al., 1999;
‡ modified from Bull et al., 1999;
§ Leeming et al., 1997
CDCA = chenodeoxycholic acid, DCA = deoxycholic acid, HDCA = hyodeoxycholic acid, LCA = lithocholic acid