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. 2017 Jan 6;12(1):e0164882. doi: 10.1371/journal.pone.0164882

Table 5. Steroid ratios for detection and identification of a faecal input applied on archaeological soil samples.

No. Ratio Dormagen Inden Düren-Arnoldsweiler
Cess-pit Stable Control Sewer Control Well
Drain (brown filling) Drain (green filling) Stable area 70 cm depth 80 cm depth LBK Bronze Age Iron Age
Ratios for mere detection of faecal matter
I (coprostanol + epicoprostanol) / (5α-cholestanol + coprostanol + epicoprostanol) 0.57 0.32 0.26 0 0 0.43 0.30 0 0.89 0.70 0.37
II (5β-stigmastanol + epi-5β-stigmastanol) / (5α-stigmastanol + 5β-stigmastanol + epi-5β-stigmastanol) 0.37 0.35 0.15 0.11 0.14 0.37 0.55 0.15 0.31 0.58 0.18
Ratios for identification of faecal matter
V coprostanol / (coprostanol + 5β-stigmastanol) x 100% § 46% 29% 39% 0% 0% 28% 18% 0% 40% 12% 42%
VI epi-5β-stigmastanol / 5β-stigmastanol + epicoprostanol / coprostanol 0.58 1.74 1.50 - - 0.50 1.42 - 0.41 2.90 1.04
Bile acid ratios
DCA / LCA 11 5.6 9.3 6.7 8.9 10 1.7 13 7.4 14.1 6.3
DCA / CDCA 8.2 6.1 9.2 9.1 9.4 11 4.5 3.2 37 24 204
CDCA / LCA 1.3 0.9 1.0 0.7 1.0 0.9 0.4 4.1 0.2 0.6 0.03
HDCA / LCA 4.6 1.2 4.3 1.3 3.5 14 0.8 11 9.5 11 1.1

All ratios calculated from the means of n = 3 laboratory replicates; when steroid contents were below the routine quantification limit, they were treated as zero for the calculation of the ratios.

References for the used ratios:

Bull et al., 1999;

modified from Bull et al., 1999;

§ Leeming et al., 1997

CDCA = chenodeoxycholic acid, DCA = deoxycholic acid, HDCA = hyodeoxycholic acid, LCA = lithocholic acid