FIG. 5.

Effects of silent mutations in SL-V and VI on in vitro translation of replicon RNA. Transcripts of WT and mutant RNAs were translated in rabbit reticulocyte lysate as described in Materials and Methods. The samples were analyzed by SDS-12.5% PAGE. The control reaction mixture (C) contained no RNA. The positions of the major translation products (NS3 and HCV core-Neo fusion protein) are indicated. The values on the left indicate the positions of molecular size standards.