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CBV4 binding is TLR4 independent. Human pancreatic (PANC-1) cells were incubated with CBV4 virions (102 PFU/ml) (white bar charts) or UV-inactivated-CBV4 (black bar charts) for 1 h. CBV4 binding was detected by using a CBV4 horse polyclonal serum followed by incubation with FITC-goat anti-horse immunoglobulin. Fluorescence was detected using a FACSCalibur system (Becton Dickinson), counting 10,000 cells per sample. The data represent the means of three independent experiments.
