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. 2004 Oct;78(20):11313–11320. doi: 10.1128/JVI.78.20.11313-11320.2004

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Copyright © 2004, American Society for Microbiology

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FIG. 8. — Energy transfer between TLR4 and CAR before and after stimulation by CBV4. Energy transfer between TLR4 (Cy3-TLR4) and CAR (Cy5-CAR) before (A and B) and after (C and D) stimulation by CBV4 can be detected by the increase in donor fluorescence after acceptor photobleaching. Donor (Cy3) images after acceptor photobleaching (A and C) and E images (images resulting from the subtraction of the Cy3 image before photobleaching from the Cy3 image after photobleaching) (B and D) are shown. Bar, 5 μm.