TABLE 2.

5′ and 3′ UTR sequence analysis and microarray transcriptional expression levels of the WSSV structural genes

VP no.	Name	WSSV ORF	TATA boxa	Inr motifb	Kozak contextc	Poly(A) signald	Transcriptional expression levele
1	VP664	WSSV419	Δ		S	+	L
2	VP180	WSSV052	Δ	+	S	+	L
3	VP136A	WSSV326	Δ	+	S		H
4	VP136B	WSSV524	+	+	W	+	M
5	VP110	WSSV092		+	S		L
6	VP95	WSSV502	Δ	+	W	+	L
7	VP75	WSSV388	Δ		S	+	M
8	VP73	WSSV275	Δ		S		L
9	VP60A	WSSV381	+		S	+	M
10	VP60B	WSSV474			W		M
11	VP55	WSSV051	Δ	+	S	+	L
12	VP53A	WSSV067		+	W	+	L
13	VP53B	WSSV171	Δ		S	+	L
14	VP53C	WSSV324	Δ	+	S		H
15	VP51A	WSSV294	Δ	+	W	+	M
16	VP51B	WSSV311	+	+	S	+	M
17	VP51C	WSSV364	+	+	W	+	H
18	VP41A	WSSV293	Δ	+	W		M
19	VP41B	WSSV298	+		S		L
20	VP39A	WSSV362	Δ	+	W		L
21	VP39B	WSSV395	+		W		L
22	VP38A	WSSV314	Δ	+	W	+	L
23	VP38B	WSSV449	Δ	+	W		L
24	VP36A	WSSV134	+	+	S	+	H
25	VP36B	WSSV309	Δ	+	S	+	H
26	VP35	WSSV019	+	+	S	+	M
27	VP32	WSSV253	Δ	+	W		L
28	VP31	WSSV396	Δ	+	W		L
29	VP28	WSSV480	Δ	+	S	+	H
30	VP26	WSSV367		+	S	+	H
31	VP24	WSSV058	Δ	+	W	+	M
32	VP22	WSSV359	+	+	S		M
33	VP19	WSSV473	+	+	S	+	H
34	VP15	WSSV269	+	+	W	+	H
35	VP13A	WSSV339		+	S		M
36	VP13B	WSSV377	Δ	+	S		M
37	VP12B	WSSV445	+		W	+	M
38	VP12A	WSSV065		+	S	+	H
39	VP11	WSSV394	Δ	+	S	+	M

^aThe presence of a TATA box (TATAAA) is indicated by +. The presence of a truncated sequence (TATA or TATAA) is indicated by Δ.

^bThe presence of an Inr motif (A/TCAC/G/TT) upstream of the translation start codon (ATG) is shown by +.

^cThe Kozak context (PuNNATGPu) of the initiation codon is either S (strong) or W (weak).

^dThe presence of a 3′ UTR poly(A) signal (AATAAA) is indicated by +.

^eMicroarray transcriptional expression levels are shown as follows: low (L), baseline mRNA levels increased by a factor of between 3 and 15; medium (M), mRNA increased by a factor of 15 to 60; high (H), increased by a factor of 60 to >300.