FIG. 2.

Cell binding of Tr- or NTr-RRV and -nar3 viruses in the presence of the recombinant proteins GST-VP8 and GST-VP5 or in the presence of MAbs to the viral proteins. (A) MA104 cells grown in 48-well plates were preincubated with 1.5 μg of affinity-purified GST-VP8 or GST-VP5 proteins. After removal of the excess of protein, 500 ng of the indicated purified virus was added. The cells were further incubated for 1 h at 4°C, and the amount of cell-bound virus was determined as described in Materials and Methods. (B) Purified viral particles (500 ng) were preincubated for 1 h at room temperature with 50 μg of the indicated MAbs to rotavirus proteins/ml. After this incubation, the virus-antibody mixture was added to a monolayer of MA104 cells, followed by incubation for 1 h at 4°C, and the amount of cell-bound virus determined. The data are expressed as the percentage of the virus bound when the cells were preincubated with PBS as a control. The arithmetic means and standard deviations for two independent experiments performed in duplicate are shown.