FIG. 7.

Binding of NTr-nar3 rotavirus to purified integrin αvβ3. (A) ELISA plates coated with αvβ3 or α5β1 integrins (100 ng per well) were incubated for 1 h at 37°C with increasing amounts of Tr- or NTr-nar3 viral particles. The virus bound to the plates was detected with a rabbit serum against rotavirus. The amount of virus added to the plate is plotted against the OD₄₉₀ reading obtained for the virus bound. (B) Different amounts of nonbiotinylated CNP or sCNP peptide were added to a 96-well plate coated with αvβ3 integrin for 1 h at 37°C. After this incubation, NTr-nar3 virus (300 ng/well) was added, followed by further incubation for 1 h at 37°C. The amount of virus bound to the plate was detected with specific antibodies to rotavirus. The data are expressed as the percentage of virus bound when the αvβ3 integrin was preincubated with PBS as a control. The arithmetic means and standard deviations of two independent experiments performed in duplicate are shown.