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. 2004 Oct;78(20):10911–10919. doi: 10.1128/JVI.78.20.10911-10919.2004

FIG. 2.

FIG. 2.

Splice junction map of SPV. Open reading frames (with ATG as the start codon) greater than 40 amino acids are indicated with boxes of different shades. Nt., nucleotide position in simian parvovirus genome (GenBank Accession U26342). ⎧, promoter site (TATAA). ⎫, site for polyadenylation (AATAAA). F, forward primer. R, reverse primer. On the left-hand side are shown the designated numbers and total lengths in nucleotides of cloned partial transcripts. The ends of these transcripts correspond to the forward and reverse primers used. Lines indicate exons, and brackets indicate spliced introns (designated alphabetically in italics). Asterisks indicate stop codons. ORFs and transcripts ending with dotted lines indicate the ends of partial transcripts corresponding to the position of the reverse primer; NS = 307 to 2370, VP1 = 2363 to 4819, and VP2 = 3149 to 4819 (6).