Skip to main content
. 2004 Oct;78(20):11233–11245. doi: 10.1128/JVI.78.20.11233-11245.2004

FIG. 4.

FIG. 4.

M84-specific CD8+-T-cell responses and immune protection after vaccination of mice with M84 DNA or M84 DNA subfragments. BALB/c mice were immunized three times i.d. with pc3Δneo (Vector), pc3-M84, pc3-M84Δ Kd, pc3-NFrg, pc3-MFrg, pc3-ΔKdMFrg, or pc3-CFrg DNA vaccines. Ten days after the last immunization, three mice per group were examined for M84-specific CD8+-T-cell responses by using the ICCS assay with either M84 297-305 peptide or M84-expressing J774 cells as stimulators. The other four mice per group were challenged with 3.5 × 105 PFU (0.5 LD50) of salivary gland-derived MCMV. Five days later, the virus titer in the spleen was determined. (A) M84-specific CD8+-T-cell responses measured by ICCS assay with M84-expressing J774 cells (left) or M84 297-305 peptide (right) as a stimulator. (B) Titer of MCMV per spleen after viral challenge. The limit of virus detection was 200 PFU per spleen, and virus titers below the detection limit were arbitrarily set to 100 PFU for display purposes and mean calculation. Each individual mouse is represented by a solid symbol.