FIG. 2.
Thin-section electron micrographs of permissive (33.5°C) and restrictive (39.5°C) cultures of WT ARV138 (A and B), tsA12 (C and D), and tsA146 (E to H). QM5 cells were infected at an MOI of 10 PFU/cell and then incubated at 33.5°C for 30 h (A, C, E, G, and H) or 39.5°C for 22 h (B, D, and F). Bar = 1.0 μm for panels A-G and 0.2 μm for the insets and panel H. (F, inset) Top component is designated by an arrowhead; the core is designated by arrows. Disruption was observed in the annulate lamallae in both permissive (G and H) and restrictive cultures of tsA146.
