TABLE 1.
Distribution of types of particles produced by ARV138, tsA12, and tsA146 at permissive and nonpermissive temperaturesa
| Clone | Temp (°C) | Total no. of particlesb | Total no. of particles/mlc | Proportion of particle type (% [SEM])d
|
No. of particlese/PFU at:
|
||||
|---|---|---|---|---|---|---|---|---|---|
| Virion | Top component | Core | Outer shell | 33.5°C | 39.5°C | ||||
| ARV138 | 33.5 | 5,273 | 2.2 × 109 | 40.4 (7.7) | 53.0 (6.0) | 3.7 (1.8) | 2.9 (0.4) | 1,038 | 1,482 |
| 39.5 | 28,127 | 7.2 × 108 | 43.7 (15.7) | 50.7 (15.5) | 2.9 (0.7) | 2.7 (0.2) | 238 | 304 | |
| tsA12 | 33.5 | 1,524 | 8.9 × 108 | 69.8 (21.2) | 18.7 (19.1) | 5.0 (2.6) | 6.5 (1.1) | 698 | 4.1 × 106 |
| 39.5 | 783 | 9.6 × 106 | 24.8 (7.5) | 35.0 (8.9) | 31.1 (18.6) | 9.1 (2.5) | 26 | 1.6 × 104 | |
| tsA146 | 33.5 | 6,923 | 4.2 × 109 | 37.8 (9.1) | 50.8 (8.6) | 6.4 (0.5) | 5.0 (0.9) | 1,049 | 1.6 × 107 |
| 39.5 | 3,185 | 5.6 × 107 | 62.8 (14.9) | 33.1 (11.38) | 2.1 (4.6) | 2.0 (1.0) | 398 | >3.5 × 105 | |
a
Calculated from three separate experiments.
b
Total number of viral and subviral particles counted.
c
Number of identifiable viral particles in cell lysates, calculated as described previously (18).
d
Proportion of indicated particle type, expressed as a percentage of the total number of particles produced, with the standard error of the mean shown in parentheses.
e
Determined for virion particles, calculated as follows: [(total no. of particles/ml × percentage of particles that are virions) ÷ titer at the indicated temperature].