TABLE 2.
Electropherotypes and EOP values of ARV176 × tsA12 reassortants
| Clone | Electropherotype of geneb
|
EOPa | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| L1 | L2 | L3 | M1 | M2 | M3 | S1 | S2 | S3 | S4 | ||
| 125 | A | A | 7 | A | A | 7 | 7 | A | 7 | 7 | 0.0000064 |
| tsA12 | A | A | A | A | A | A | A | A | A | A | 0.000045 |
| 131 | 7 | 7 | 7 | 7 | A | A | A | A | A | A | 0.000068 |
| 187 | A | A | A | 7 | A | A | A | A | A | A | 0.000094 |
| 160 | 7 | 7 | 7 | A | 7 | 7 | 7 | A | A | 7 | 0.000096 |
| 192 | A | A | 7 | 7 | A | A | A | A | A | A | 0.00025 |
| 26 | 7 | A | 7 | 7 | A | 7 | 7 | A | A | 7 | 0.00034 |
| 186 | A | A | A | 7 | A | A | 7 | Ac | 7 | A | 0.00069 |
| 151 | A | 7 | 7 | 7 | 7 | A | 7 | A | A | 7 | 0.00081 |
| 64 | A | 7 | 7 | A | A | A | A | A | A | A | 0.0011 |
| 220 | 7 | A | A | A | A | 7 | 7 | Ac | 7 | A | 0.0025 |
| 222 | A | A | 7 | 7 | 7 | A | A | Ac | 7 | A | 0.0040 |
| 12 | A | A | 7 | A | 7 | A | 7 | A | A | A | 0.0043 |
| 169 | 7 | 7 | 7 | 7 | A | 7 | 7 | 7 | 7 | A | 0.19 |
| 65 | 7 | 7 | 7 | 7 | A | A | 7 | 7 | 7 | 7 | 0.27 |
| 229 | A | 7 | 7 | 7 | A | 7 | A | 7 | 7 | A | 0.41 |
| 30 | 7 | A | 7 | 7 | A | 7 | 7 | 7 | 7 | A | 0.53 |
| 148 | 7 | A | 7 | 7 | A | A | 7 | 7 | 7 | 7 | 0.55 |
| 215 | 7 | 7 | 7 | 7 | 7 | A | 7 | 7 | 7 | 7 | 0.69 |
| 243 | 7 | 7 | 7 | 7 | A | 7 | 7 | 7 | 7 | 7 | 0.74 |
| ARV176 | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 0.76 |
| 194 | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 | A | 7 | 1.42 |
| 159 | 7 | 7 | 7 | 7 | 7 | 7 | A | 7 | 7 | 7 | 20.52 |
| Exceptions | 5 | 6 | 9 | 7 | 10 | 7 | 9 | 0 | 5 | 7 | |
a
EOP, efficiency of plating [(titer at 39.5°C) + (titer at 33.5°C)]. Values represent the averages obtained from two or three experiments.
b
A, tsA12; 7, ARV176.
c
Gene segment exhibits an aberrant mobility in 12.5% polyacrylamide gels, and the parental origin was identified by restriction fragment length polymorphism analysis.