TABLE 1.

Sequences of the oligonucleotides used in this study

Primer no.	Primer namea	Sequence (5′→3′)
1	G129V-for	CGCTGGAGCGGCTGTTGTCAGCATAGGCCTTGGGAAGG
2	G129V-rev	CCTTCCCAAGGCCTATGCTGACAACAGCCGCTCCAGCG
3	I131N-for	GCGGCTGTTGGCAGCAACGGCCTTGGGAAGGTGC
4	I131N-rev	GCACCTTCCCAAGGCCGTTGCTGCCAACAGCCGC
5	K135S-for	GCAGCATAGGCCTTGGGAGTGTGCTTGTGGATATTTTGG
6	K135S-rev	CCAAAATATCCACAAGCACACTCCCAAGGCCTATGCTGC
7	K135R-for	GCAGCATAGGCCTTGGGAGGGTGCTTGTGGATATTTTGG
8	K135R-rev	CCAAAATATCCACAAGCACCCTCCCAAGGCCTATGCTGC
9	3800sp-for	GTCATTGTGGGCAGGATCATCTTGTCCGGAAAGCC
10	5Right-rev	GTGACCCAACCAGGTATATTGATTGAGCCCGACCAGGAATGTGACC
11	Ncol-4B-for	CAGCCATGGCCTCACACCTCCCTTACATCG
12	4B-NcoI-rev	CATGCCATGGCGCATGGCGTGGAGCAGTCCTCG
13	Attb-TEV-4B-for	GGGGACAAGTTTGTACAAAAAAGCAGGCTTCGAAAACCTGTATTTTCAGGGCGCCTCACACCTCCCTTACATCGAAC
14	4B-stop-attb-rev	GGGGACCACTTTGTACAAGAAAGCTGGGTTTAGCATGGCGTGGAGCAGTCCTCG
15	4Left-for	AGAGCGTCTTTACAGGCCTCACCCACATAGACGCCCATTTCTTGTCCCAG
16	4Right-rev	AGGGCGCCAGGGGAGAGGATAGCAGGGAGTAGGTTAACCAGGTCCTCGG

^afor, forward primers; rev, reverse primers.