FIG.4.

Decreased production of TNF-α in effector but not memory cells from mice treated with MnTBAP during acute viral infection. C57BL/6 mice were treated with either the vehicle or 5 mg of MnTBAP/kg and then infected with LCMV Armstrong. A maintenance dose was administered every 24 h from days 0 to 8. Mice were sacrificed on days 5 (A), 8 (B), or 38 (C) postinfection, and splenocytes were stimulated with the indicated LCMV epitope. Cells were then stained with anti-CD8α, IFN-γ, and TNF-α. The dot plots are gated on CD8⁺ T cells, and the number in the plot indicates the percentage of CD8⁺ T cells that are present in that region. Six to 10 mice were analyzed at each time point, and results for a representative mouse are shown. To determine the effect of MnTBAP on cytokine production (D), splenocytes were isolated from a mouse 8 days postinfection with LCMV Armstrong and were incubated with NP396-404 and increasing concentrations of MnTBAP. The numbers of cytokine producing CD8⁺ T cells were quantitated, and the average and standard deviation are shown. Six mice were analyzed in two independent experiments.