FIG. 3.
HCMV viral growth curves. LEHFFs were infected under normal conditions (−Rap, +serum), under hypoxic conditions (−O2, +serum), and in the presence of rapamycin (+Rap, +serum) and harvested at various times after infection up to 144 h. Virus titers at each time point were determined by the 50% tissue culture infective dose method. In addition, viral growth curves were generated under conditions where the cells were serum starved for 48 h and then infected and maintained in the absence of serum (−Rap, −serum) or in the absence of serum with the addition of rapamycin (+Rap, −serum).