FIG. 2.

LMP2A deletion mutants are palmitoylated. EBV-negative BJAB B cells were transiently transfected with full-length and LMP2A deletion constructs (shown in panel A). After 4 h, the cells were incubated with [³H]palmitic acid (³H-Palm) for an additional 4 h and then lysed. Lysates were immunoprecipitated for metabolically labeled LMP2A followed by SDS-PAGE and autoradiography (B, top). Western blot analysis was performed in parallel with antibodies to LMP2A (αLMP2A) (B, bottom). Each deletion mutant maintained palmitoylation like full-length LMP2A, whereas no bands were detected in the pSG5 vector control lane. The data shown represent at least three independent experiments.