TABLE 2.

Quantification of primer extension products^a

FL or RL	Amt. of product
	WT	TA28m	TA29.1m	TA29.2m	TA29.3m
29FL upper	12.78 ± 2.67	12.68 ± 3.89	2.92 ± 2.03	107.79 ± 21.6	2.12 ± 1.33
29FL lower	100	108.28 ± 11.88	120.09 ± 6.77	1.88 ± 1.58	1.33 ± 0.77
28RL	100	1.10 ± 0.78	98.22 ± 25.47	149.27 ± 36.93	60.14 ± 13.91

^aThe primer extension products from MeWo cells transfected with pRFL/WT, pRFL/TA28m, pRFL/TA29.1m,pRFL/TA29.2m, and pRFL/TA29.3m were quantified by phosphorimage analysis. The upper and lower bands of 29 FL extension products were analyzed in the same group for comparison. In the case of the 29 FL extension products, the level of expression observed with the lower (110-base) product in the wild-type (WT) promoter was normalized to 100%. In the case of the 28 RL reporter, the total level of expression present in both bands was normalized to 100%. Shown here are the averages and standard deviations derived from three independent experiments.