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. 2004 Oct;78(20):11007–11015. doi: 10.1128/JVI.78.20.11007-11015.2004

FIG. 6.

FIG. 6.

Synthesis and localization of viral proteins in virus-infected MDCK cells. (A) Time course of viral protein synthesis of BM2stop mutant and rg-B/Y wt viruses. Viral proteins were detected by Western blotting with either anti-B/Yamagata (upper panel) or anti-BM2 (lower panel) antibody. Lane M is the lysate of uninfected cells. (B and C) Intracellular localization of the viral proteins of BM2stop mutant and rg-B/Y wt viruses. At 11 hpi, infected cells were fixed and subjected to IFA. Panels show confocal microscopy images of XZ sections, with the apical membrane uppermost. (B) BM2 (red) and HA (red) were detected together with β-catenin (green). β-Catenin is located at the lateral membrane. (C) NP (red) and M1 (red) were detected together with HA (green).